research programs
medical thesis program
Ph.D. program
protocols
Protocols
(restricted to Lab members)

RNA technology
Electrophoretic mobility shift assay, PCR genotyping, RNA isolation from cells, RNA isolation from tissues, RNAse protection assay, real-time RT-PCR, in-situ hybridization, siRNA, northern blot

Protein technology
Western blot, ELISA, 4-color flow cytometry, immunoprecipitation assay

Molecular biology
Generation of transfection vectors, transient transfection, siRNA

Cell culture
leukocyte isolation from the nephritic kidney, preparation of primary renal cells, preparation of immune cell subsets, culture conditions for established cell lines, ECIS analysis system

Histology
Tissue fixation, frozen sections, paraffin-embedded sections, immunohistochemistry, immunofluorescence, established antibodies for immunostaining, digital morphometry, manual morphometry, laser confocal microscopy, laser microdissection

Animal care and experimentation
Housing conditions, health certificates, anaesthesia protocol, microsurgery

Procedures
Irradiation and bone marrow transplantation, ex vivo cell labeling, cell-transfer studies

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Dr. H.-J- Anders, Dr. V. Vielhauer, anders & vielhauer, research laboratory, research interests, research programs, medical thesis program, Ph. D. program, publications, protocols, Leukocyte activation, Renal cell activation, Leukocyte migration, Target identification program, Target validation program, Drug validation program in disease models, Clinical Science